Glycine Titration Curve And Its Steps

Glycine is an amino acid, building blocks of protein. It is not basically an “essential amino acid”, because the body can produce it from other chemicals. A typical diet constitutes about 2 grams of glycine daily. The primary sources are protein-rich foods incorporating, meat, fish, dairy, and legumes.

Glycine is utilized for piss up schizophrenia, stroke, benign prostatic hyperplasia (BPH), and some uncommon inherited metabolic disorders. It is also used to prevent kidneys from the harmful side effects of different drugs used after organ transplantation as well as the liver from dangerous effects of alcohol. Other uses incorporates cancer prevention and memory enhancement.

glycine titration curve

How does it work?

The body utilizes glycine to built proteins. Glycine is also play a role in the transmission of chemical signals within the brain, so there is interest in treating it for schizophrenia and enhancing memory. Some researchers realize glycine may have a crucial role in cancer prevention, because it seems to interfere with the blood supply required by incontestable tumors.

What are the risks of taking glycine?

Glycine considers to be safe, even at doses as high as 60 grams per day. But glycine’s safety has not been fully tested. Obvious caution should be used when considering glycine for young children, pregnant or breastfeeding women, and the people having a diseases of kidney or liver.

Instead, of being people are treating with clozapine and avoiding the treat with glycine. Also people who have had a stroke problem should not take glycine without the recommendation of a doctor.

A lots of people have consulted with issue of nausea, vomiting, and upset stomach after in-taking glycine. Such reports have been unique, and the risks have gone away after glycine was discontinued.

Steps of Titration curve of Glycine:


At very much low pH, the usually ionic species of Glycine is the completely protonated form, +H3N-CH2-COOH. For Glycine, the pH at the middle is 2.34, thus it’s –COOH group has pKa of 2.34 which is generally high.


As the titration conveys, another crucial stage is touched at pH 5.97. However, there is one more point of infection, at which removal of the very first proton is essentially achieved and discharge of the second proton has just began.

Stage 3:

The third stage of the titration be consonant with to the discharge of proton from the –NH3+ group of Glycine. The pH at the mid level of this point is 9.60, the equal to the pKa for the –NH3+ chain.

From this titration curve of glycine we can derive several important pieces of information.

  • It provides quantitative measure of the pKa of every of the couple of ionic groups; 2.34 for the –COOH group and 9.60 for the –NH3+ group.
  • The unsettled pKa of Glycine is caused by continuous repulsion in between the departing proton and the close by positively charged amino group on the α-carbon atom, the repulsion can be vigorous.
  • The titration curve of Glycine has a couple of portions of buffering power. At pKa 2.34, glycine is a better buffer close to this pH. The further buffering zone is centered on pH 9.60.
  • Glycine is not an effective buffer at the pH of intracellular fluid (or) blood, about 7.4 ph. To measure the buffering ranges, we can take in use Handerson-Hasselbalch equation.

Hi, This is Hamza Khan from Peshawar, Pakistan. I am a 2nd prof MBBS Student at Bannu Medical College and a hobby Blogger. The Purpose of this site is to share my knowledge and Guide new Medical Students.

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