Haemocytometer is an apparatus used to count various blood cells (RBC , WBC and a easinophll and platelets). It consists of RBC and WBC pipette (Thoma diluting pipettes) thick slide (Neubauer's chamber). REAGENTS: Hayem's fluid for RBC counting and its composition: NaCl 0.5 g for … [Read more...]
estimation of total leukocyte count (TLC):
APPARATUS Neubauer’s chamber. WBC pipette (Thomas diluting pipette), diluting fluid cover slip, pricking needle (blood lancet) and spirit swab. METHODS: There are two methods of calculating the WBC count: Electronic counting method(Computerized method). Visual method. WBC … [Read more...]
Study Effect Exercise Blood Pressure:
THEORY To study the effect of exercise on BP, it is very important to know the severity exercrse. The grading of exercise is done depending upon percentage Of 02 used in relation to V02 max during exercise (V02 max is the rate of Oz consumtion during strenous exercise by the … [Read more...]
Clinical Examination Arterial Pulse:
Arterial pulse can be examined (palpated) in main peripheral arteries the radial, brachial, carotid, femoral, popliteal, posterior tibial and dorsalis pedis. Redial pulse examination is preferred as it can be easily felt. It is best felt with the tips of the three fingers, slightly compressing the … [Read more...]
Osmotic Fragility Erythrocytes Test:
APPARATUS (Specimen and Reagents) 15 small test tubes, disposable syringe, rack for holding tubes, long nozzle dropper, 0.5% NaCl solution, fresh heparinized blood and distilled water. PRINCIPLE: It is designed as the ease with which the cells are broken down in hypotonic solutions. It is … [Read more...]