THEORY. Precordium is the portion of the anterior aspect of the chest wall which overlies the heart. Its examination is carried out under following headings: Palpation Inspection percussion. Auscultation Inspection Clothing of the patient should be removed upto waist. … [Read more...]
Blood Pressure Measurement Method:
PRINCIPLE : The principle of the experiment involves the balancing of air pressure against the pressure of blood in brachial artery, the air pressure being estimated by a mercury or air (aneroid) manometer . A cloth-covered rubber bag is wrapped around upper arm, and is inflated with air till … [Read more...]
Differential Leukocyte (WBC) Count Procedures:
APPARATUS (Reagents and equipments): 4-5 clean glass slides, Leishman’s stain, blood lancet, dropper, glass rod, compound microscope, cedar wood oil, buffered water and staining tray. PRINCIPLE: A blood film appropriately prepared and stained is seen under the microscope in oil immersion. … [Read more...]
Blood Smear (blood film) Preparation Procedure & Staining:
The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman's stain, wash … [Read more...]
Determination Red Blood Cell Count Method:
Apparatus: Neubauer’s chamber (thick slide), RBC diluting fluid, microscope, coverslip, pricking needle (blood lancet) and spirit swab. RBC Diluting Fluid (Hayem’s Fluid): Sodium chloride (NaCl): 0.5 gm, to maintain isotonicity of fluid. Sodium sulphate (Na2504): 2.5 gm, which breaks … [Read more...]