Arterial pulse can be examined (palpated) in main peripheral arteries the radial, brachial, carotid, femoral, popliteal, posterior tibial and dorsalis pedis. Redial pulse examination is preferred as it can be easily felt. It is best felt with the tips of the three fingers, slightly compressing the … [Read more...]
Osmotic Fragility Erythrocytes Test:
APPARATUS (Specimen and Reagents) 15 small test tubes, disposable syringe, rack for holding tubes, long nozzle dropper, 0.5% NaCl solution, fresh heparinized blood and distilled water. PRINCIPLE: It is designed as the ease with which the cells are broken down in hypotonic solutions. It is … [Read more...]
Packed Cell Volume (PCV) Test Procedure: Hematocrit:
APPARATUS: Wintrobe’s tube with stand, 2 ml disposable syringe with needle, Pasture pipette, mixture of double oxalate, centrifuge machine and spirit swab. PRINCIPLE: Anticoagulant mixed blood is filled in a tube and centrifuged. Cells settle down towards the bottom because of their greater … [Read more...]
Blood (ABO) Group Typing Test [PROCEDURE]:
Apparatus: Normal saline, antisera A, B & D, pricking needle (blood lancer); porcelin tile/glass slides, dropper, application sticks or tooth picks, glass marking pencil, cover slips, microscope and spirit swab. PRINCIPLE Blood Group Test: Blood group means the presence of antigen on RBCs … [Read more...]
Hemoglobin Test; How to Perform:
Apparatus: Hemoglobinometer, pricking needle (blood lancet) and spirit swab. Sahli’s method: Hemoglobinometer: It is a box which contains: Stirrer: Simple glass rod used to mix the blood with acid or distilled water. Hemoglobin pipette: it is a simple pipette having capillary tube in … [Read more...]