The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman's stain, wash … [Read more...]
Archives for February 2017
Determination Red Blood Cell Count Method:
Apparatus: Neubauer’s chamber (thick slide), RBC diluting fluid, microscope, coverslip, pricking needle (blood lancet) and spirit swab. RBC Diluting Fluid (Hayem’s Fluid): Sodium chloride (NaCl): 0.5 gm, to maintain isotonicity of fluid. Sodium sulphate (Na2504): 2.5 gm, which breaks … [Read more...]