Titrable acidity of urine is expressed as the amount ofalkali required to titrate or neutralize the acid present in the urine. The_titrable acidity, in the normal urine, is mainly due to the presence of acid phosphate ions (H2P04 ) and to a small extent due to organic acids (Urates, oxalates and … [Read more...]
Archives for May 2017
Volhard-Harvey test: Estimation of chloride in urine:
Chloride concentration of urine is dependent upon the amount of chloride present in the ingested food. Volhard-Harvey Method: PRINCIPLE: The chloride in the urine is precipitated as silver chloride with a measured excess of silver nitrate The excess of silver nitrate is treated With ammonium. … [Read more...]
Benedict’s Test: Estimation Glucose in urine:
The amount of glucose present in the urine of a normal subject is about 100mg/day, which is not detectable by the commonly employed qualitative methods. However, in some persons glucose may appear in the urine as in benign glucosuria which is not uncommon. METHOD: Benedict’s … [Read more...]
estimation of total leukocyte count (TLC):
APPARATUS Neubauer’s chamber. WBC pipette (Thomas diluting pipette), diluting fluid cover slip, pricking needle (blood lancet) and spirit swab. METHODS: There are two methods of calculating the WBC count: Electronic counting method(Computerized method). Visual method. WBC … [Read more...]
Estimation serum Creatinine By Folin’s Method (Jaffe’s Reaction)
Creatinine is an anhydride of creatine. It is formed in the muscle from creatine phosphate by an irreversible, non-enzymatic dehydration and loss of phosphate. Serum creatinine (a blood measurement) is an important indicator of renal health because it is an easily measured byproduct of muscle … [Read more...]